Shibu holds a Bachelors degree in Biotechnology from Vellore Institute of Technology, Vellore, India and a Masters degree from the Université de Paris. During his masters, he completed 3 internships in the first one on building genetic circuits to control autophagy, the second one in the field of neuroscience to map the functional connectivity in the social interaction network of monkey brains and the last one on understanding Transcription factors networks driving ageing in Cockayne syndrome. His current project involves developing a time-resolved proteome map of the ciliary proteome.

Abstract
Cilia disassembly is a crucial process for cells to enter the cell cycle and proliferate but also for the function of certain cell types (i.e. retinal pigment epithelial cells). In contrast to ciliary assembly, disassembly is only partially understood and especially the timing of disassembly induction and execution is unclear. In this study, we aim at identifying the protein networks, involved in ciliary disassembly in a time-resolved fashion to understand both the mechanisms of initiation and execution of this process. We will use affinity- and proximity-based methods to quantitatively study the network by analysing the protein complexes of key players and by defining the protein repertoire of cilia at different stages. CRISPR/Cas9 will be used to endogenously tag bait proteins, TurboID, as well as FLAG-based affinity enrichment, will be used to quantitatively analyse the complexes using high-resolution mass spectrometry and statistical analysis of the quantitative data. The initial results will be complemented by alternative proteomic approaches and by localization studies using superresolution microscopy (gSTED) and live-cell imaging. The data will be integrated into the existing ciliary landscape and analysed to define testable hypothesis that will be validated in differentiated retinal pigment epithelial cells.